MHC class II expression by mast cells in the genital tract of cows

This work examined the presence of MHC class II molecules expressing mast cells in oviduct, uterus and vaginal tissues in cows. The tissue samples of five cows were collected from a local slaughterhouse. Toluidine blue pH 0.5 (TB) and avidin-biotin peroxidase complex (ABC) staining procedures applied to adjacent sections from tissue samples. It was determined that some TB + cells were also gave positive reaction with strept ABC staining for MHC II molecules. To our knowledge this is the first evidence indicating the presence of MHC class II molecules expressing mast cells in the cow.