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Endothelin-1-like immunoreactivity in human urine.
1Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Nephron
|January 1, 1991
Summary
Endothelin-1 (ET-1) is present in human urine, with significant daily excretion measured in normal subjects. This study confirms urinary ET-1 excretion, though its renal source and function require further investigation.
Area of Science:
- Nephrology
- Endocrinology
- Biochemistry
Background:
- Endothelin-1 (ET-1) is a potent vasoconstrictor peptide.
- The presence and role of ET-1 in renal physiology are not fully understood.
- Investigating urinary ET-1 can provide insights into renal function and ET-1 metabolism.
Purpose of the Study:
- To determine the presence and quantify endothelin-1-like immunoreactivity (ET-1-LI) in human urine.
- To compare urinary ET-1-LI excretion with plasma ET-1-LI levels.
- To characterize the nature of ET-1-LI in urine.
Main Methods:
- Radioimmunoassay (RIA) specific for ET-1 was used to measure ET-1-LI.
- Urinary ET-1-LI was measured in daily collections and spot urine samples.
- Plasma ET-1-LI concentrations were measured in the same subjects.
- Reverse-phase high-pressure liquid chromatography (RP-HPLC) was employed for characterization.
Main Results:
- Significant amounts of ET-1-LI were detected in human urine.
- Daily urinary excretion of ET-1-LI was 67.6 +/- 35.5 ng/day in normal subjects.
- Urinary ET-1-LI (82.8 +/- 38.2 pg/mg creatinine) did not correlate with plasma ET-1-LI (1.1 +/- 0.5 pg/ml).
- RP-HPLC confirmed a major ET-1-LI component in urine coeluting with standard ET-1.
Conclusions:
- Endothelin-1-like immunoreactivity is excreted in human urine.
- Urinary ET-1-LI levels are independent of plasma ET-1-LI concentrations.
- The origin and physiological significance of urinary ET-1 in renal tissues warrant further research.