Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Inner Mitochondrial Membrane

The Inner Mitochondrial Membrane

The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Activity-Dependent Regulation of Basal Forebrain Cholinergic Neurons by Striatal Spiny Projection Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same author

Genetic modulation of mitochondrial NAD+ regeneration does not prevent dopaminergic neuron dysfunction caused by mitochondrial complex I impairment.

Frontiers in cell and developmental biology·2025

Same author

The many ways to inhibit translation by Sorafenib in liver cancer cells.

Molecular and cellular biochemistry·2025

Same author

Genetic modulation of mitochondrial NAD+ regeneration does not prevent dopaminergic neuron dysfunction caused by mitochondrial complex I impairment.

bioRxiv : the preprint server for biology·2025

Same author

Sorafenib-associated translation reprogramming in hepatocellular carcinoma cells.

RNA biology·2025

Same author

α-Synuclein pathology disrupts mitochondrial function in dopaminergic and cholinergic neurons at-risk in Parkinson's disease.

Molecular neurodegeneration·2024

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Mitochondria: the calcium connection.

Laura Contreras¹, Ilaria Drago, Enrico Zampese

¹Department of Biomedical Sciences, University of Padua, Italy. lcontreras@bio.unipd.it

Biochimica Et Biophysica Acta

|May 18, 2010

Summary

This summary is machine-generated.

Mitochondria manage cellular calcium, impacting energy, cell signaling, and apoptosis. New genetically encoded tools offer high-definition insights into mitochondrial calcium dynamics and its role in diseases like Alzheimer's.

More Related Videos

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Published on: April 27, 2018

Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

Published on: May 1, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Published on: April 27, 2018

Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

Published on: May 1, 2018

Area of Science:

Cell Biology
Mitochondrial Physiology
Biochemistry

Background:

Mitochondrial calcium handling is crucial for cellular energy production, calcium buffering, and apoptosis.
Despite extensive research, challenges remain in understanding mitochondrial calcium homeostasis.
Technical advancements are continuously opening new avenues for studying calcium dynamics.

Purpose of the Study:

To present a state-of-the-art toolkit for calcium measurements, emphasizing genetically encoded indicators.
To summarize key features of cellular and mitochondrial calcium handling.
To highlight recent breakthroughs and the role of calcium in pathophysiology, using Alzheimer's disease as an example.

Main Methods:

Review of current literature and technologies for calcium measurement.
Focus on genetically encoded calcium indicators for targeted sub-cellular measurements.
Discussion of techniques for characterizing mitochondrial calcium dynamics and microdomains.

Main Results:

Genetically encoded indicators allow for high-definition, targeted studies of calcium dynamics.
Recent studies have characterized calcium microdomains on the mitochondrial surface.
Alterations in calcium homeostasis, particularly mitochondrial, are implicated in Alzheimer's disease.

Conclusions:

Advanced tools, especially genetically encoded indicators, are revolutionizing the study of mitochondrial calcium.
Understanding mitochondrial calcium handling is vital for cellular function and disease mechanisms.
Further research into calcium dysregulation in neurodegenerative diseases like Alzheimer's is warranted.