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Related Experiment Video

Updated: Jun 13, 2026

Extraction of Tissue Antigens for Functional Assays
08:32

Extraction of Tissue Antigens for Functional Assays

Published on: September 10, 2012

A method for extracting tissue proteins for use in lymphocyte function assays.

Hayley C Moon1, Max Joffe, Helen E Thomas

  • 1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 9 Princes St, Fitzroy, Victoria 3065, Australia.

Journal of Immunological Methods
|May 18, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new method to extract proteins from human tissues. This technique yields non-toxic tissue lysates, enabling the analysis of human T-cell responses for transplantation and cancer research.

Area of Science:

  • Immunology
  • Biochemistry
  • Proteomics

Background:

  • Assessing human T-cell responses to tissue extracts is challenging due to toxic byproducts from conventional cell lysis methods.
  • Existing protocols are incompatible with functional T-cell assays, limiting research in areas like autoimmunity and transplantation.

Purpose of the Study:

  • To optimize a protein extraction protocol for human tissues that yields non-toxic lysates.
  • To enable the functional analysis of human T-cell responses to tissue-derived proteins.

Main Methods:

  • Human tissues were homogenized in a butan-1-ol, acetonitrile, and water mixture, followed by lyophilization.
  • Lyophilized protein extracts were dissolved in 8M urea, a concentration found to be non-inhibitory to T-cell function.
  • The optimized method was validated using islet, acinar, and spleen tissue extracts.

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Last Updated: Jun 13, 2026

Extraction of Tissue Antigens for Functional Assays
08:32

Extraction of Tissue Antigens for Functional Assays

Published on: September 10, 2012

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
07:29

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

Published on: December 6, 2014

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Main Results:

  • The developed protocol successfully generated human tissue lysates compatible with T-cell functional analysis.
  • Cytokine production and proliferation responses were detected from various human tissue extracts.
  • The urea concentration used (8M for extraction, <0.08M in assay) did not impair T-cell functionality.

Conclusions:

  • This optimized method allows for the rapid preparation of human tissue lysates suitable for T-cell response analysis.
  • The technique facilitates the study of adaptive immune responses in transplantation, cancer, and autoimmunity.
  • This approach overcomes previous limitations posed by toxic cell lysis byproducts.