Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guiding Principles: Current Practices and Considerations for Benchmarking Human Gastrointestinal Organoids.

Cellular and molecular gastroenterology and hepatology·2026
Same author

IRTKS promotes Tir membrane insertion for intimate bacterial attachment and subsequent pedestal formation.

bioRxiv : the preprint server for biology·2026
Same author

A role for LIMA1 in the assembly of actin bundle-supported protrusions.

Molecular biology of the cell·2026
Same author

EPS8 dampens the growth dynamics and prolongs the lifetime of actin-based protrusions.

bioRxiv : the preprint server for biology·2026
Same author

Asymmetric branched F-actin networks at apical clathrin coated pits support microvillar biogenesis.

Molecular biology of the cell·2026
Same author

Spef1 is a microvillar component that limits apical actomyosin contractility and preserves intestinal barrier function.

Current biology : CB·2026

Related Experiment Video

Updated: Jun 13, 2026

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
11:55

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Published on: July 12, 2022

Leveraging the membrane - cytoskeleton interface with myosin-1.

Russell E McConnell1, Matthew J Tyska

  • 1Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37205, USA.

Trends in Cell Biology
|May 18, 2010
PubMed
Summary

Class 1 myosins are motor proteins that interact with cell membranes and actin. Their unique properties allow them to regulate membrane-cytoskeleton dynamics and respond to physical forces.

More Related Videos

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

Related Experiment Videos

Last Updated: Jun 13, 2026

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
11:55

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Published on: July 12, 2022

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Class 1 myosins are motor proteins that bind to actin filaments and cellular membranes.
  • They are involved in crucial cellular processes like endocytosis and exocytosis.

Purpose of the Study:

  • To highlight the unique properties of Class 1 myosins.
  • To emphasize their role in regulating membrane-cytoskeleton interactions and responding to mechanical forces.

Main Methods:

  • Literature review of recent studies on Class 1 myosins.
  • Analysis of their mechano-chemical properties and cellular functions.

Main Results:

  • Class 1 myosins generate mechanical force at the membrane-cytoskeleton interface.
  • They play roles in endocytosis, exocytosis, and extracellular vesicle release.
  • Many exhibit load-dependent cycles, maintaining tension without ATP hydrolysis.

Conclusions:

  • Class 1 myosins are key regulators of dynamic membrane-cytoskeleton interactions.
  • Their ability to sense and respond to physical forces positions them uniquely in cellular mechanics.