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Related Experiment Video

Updated: Jun 13, 2026

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
09:47

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

Published on: September 13, 2018

An international randomized multicenter comparison of nasal potential difference techniques.

George M Solomon1, Michael W Konstan, Michael Wilschanski

  • 1University of Alabama at Birmingham, Birmingham, AL 35294-0006, USA.

Chest
|May 18, 2010
PubMed
Summary

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A new agar nasal catheter improves cystic fibrosis transmembrane conductance regulator (CFTR) testing reliability. This nonperfusing method reduces artifacts, offering a more dependable way to measure CFTR activity in clinical trials.

Area of Science:

  • Respiratory Medicine
  • Medical Device Technology

Background:

  • Transepithelial nasal potential difference (NPD) assesses cystic fibrosis transmembrane conductance regulator (CFTR) activity.
  • Current NPD testing methods suffer from unreliability and artifacts, limiting their diagnostic and clinical trial utility.

Purpose of the Study:

  • To compare the reliability and artifact susceptibility of a nonperfusing (agar gel) nasal catheter versus a continuously perfusing nasal catheter for NPD measurement.
  • To evaluate the impact of catheter type on CFTR activity assessment.

Main Methods:

  • A multicenter, randomized, crossover trial involving 26 healthy adults.
  • Standardized NPD protocol applied using both agar gel and continuously perfusing nasal catheters.
  • Identical data capture techniques were used for both methods.

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Last Updated: Jun 13, 2026

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
09:47

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

Published on: September 13, 2018

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
08:55

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

Published on: July 4, 2018

Main Results:

  • The agar catheter significantly reduced artifact frequency by 75% and duration.
  • Sodium conductance measurements were comparable between catheter types.
  • Agar catheter demonstrated significantly greater CFTR-dependent hyperpolarization (224.2 ± 12.9 mV vs. 18.2 ± 9.1 mV).

Conclusions:

  • The agar nasal catheter offers superior reliability for NPD measurement compared to perfusion methods.
  • This nonperfusion technique is a promising standardized protocol for monitoring CFTR activity in clinical trials.