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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 13, 2026

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Published on: November 7, 2019

High-sensitive fluorescence endoscope using electrocardiograph-synchronized multiple exposure.

Takehiro Ando¹, Kazuhiro Taniguchi, Hongho Kim

¹Graduate School of Engineering, The University of Tokyo, Engineering Building No 14, Room 722, Hongo 7-3-1, Bunkyo, Tokyo, Japan. take_and_o@bmpe.t.u-tokyo.ac.jp

International Journal of Computer Assisted Radiology and Surgery

|May 18, 2010

Summary

This summary is machine-generated.

A new fluorescence endoscope uses electrocardiograph (ECG)-synchronized multiple exposure (ESME) to create bright cardiac images during surgery. This technique overcomes light limitations for improved intraoperative examination of heart conditions.

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Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

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Published on: November 7, 2019

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

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Published on: March 8, 2017

Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Dual-Dye Optical Mapping of Hearts from RyR2^R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Published on: December 22, 2023

Area of Science:

Medical imaging
Cardiovascular surgery
Biophotonics

Background:

Autofluorescence imaging of the heart is limited in endoscopic surgery due to insufficient light transmission.
Existing autofluorescent substances in the heart are not optimally utilized for diagnostic purposes during procedures.

Purpose of the Study:

To develop a highly sensitive fluorescence endoscope for cardiac disease visualization.
To overcome the limitations of light transmission in endoscopic surgery for cardiac imaging.

Main Methods:

Developed an electrocardiograph (ECG)-synchronized multiple exposure (ESME) system.
Integrated an endoscope, excitation light, ECG amplifier, trigger/delay unit, and computer.
Utilized the heart's periodic motion and ECG triggering to synthesize bright images from multiple exposures.

Main Results:

The ESME system successfully generated high-quality cardiac images synchronized with heart rate.
Image quality was minimally affected by organ displacement, estimated at 0.65 mm.
Achieved bright fluorescent imaging in a low illumination environment.

Conclusions:

A novel, highly sensitive fluorescence endoscope employing the ESME approach was successfully developed and validated.
The system enables high-quality image acquisition in low-light conditions, ideal for faint fluorescence detection.
This technology is valuable for intraoperative assessment of cardiac status during surgery.