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Updated: Jun 13, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
Juan Pablo Riva Mercadal1, Paula Isaac, Faustino Siñeriz
1Planta Piloto de Procesos Industriales Microbiológicos PROIMI-CONICET Av Belgrano y Caseros T4001MVB Tucumán, Argentina.
Abstract:
A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 degrees C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min(-1) mg dry biomass(-1), obtaining 75.5 microM of indigo after 8 h of incubation, while a maximal concentration (138.1 microM) of indigo was obtained after 20 h.
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