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Updated: Jun 13, 2026
12:32
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
Published on: December 14, 2019
The characterization of plasmids in the enterobacteria
The Journal of Hygiene
|May 18, 2010
Summary
This study details routine methods for analyzing enterobacterial plasmids in a reference laboratory. These techniques effectively categorize plasmids from various sources, aiding in microbial research.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Enterobacterial plasmids are crucial genetic elements in bacterial evolution and pathogenesis.
- Standardized methods are essential for accurate plasmid characterization in diagnostic and research settings.
Purpose of the Study:
- To describe the routine methodologies employed at the Enteric Reference Laboratory for enterobacterial plasmid analysis.
- To evaluate the utility of these methods for categorizing diverse plasmids.
Main Methods:
- Description of standard operating procedures for plasmid isolation and characterization.
- Application of established techniques to a collection of enterobacterial plasmids.
Main Results:
- The described routine methods are effective for studying enterobacterial plasmids.
- Successful application of these methods allowed for the categorization of plasmids based on their origin and characteristics.
Conclusions:
- The laboratory's routine methods provide a valuable framework for enterobacterial plasmid analysis.
- These validated techniques support the effective categorization and understanding of plasmid diversity.
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