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Updated: Jun 13, 2026

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
Published on: January 25, 2017
Comparison of methods of detecting bacterial biofilm
Yutaka Tajima1, Tatsuo Yamaguchi, Rie Takagi
1Legal Medicine and Molecular Genetics, Postgraduate School of Medicine, Gunma University, Maebashi 371-8511, Japan. y-tajima@koaikai.or.jp
Abstract:
Fresh bacterial biofilm produced by Staphylococcus epidermidis was sensitively detected by using a chemiluminescent probe. Its lower detection limit was around 10(-5) - 10(-4) dilution (equivalent to 10(-5) - 10(-4) cm2 biofilm) - 10(2) - 10(3) times as sensitive as ordinary colorimetric methods. The linearity and dynamic range were slightly better than those of fluorometry.
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