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Updated: Jun 13, 2026

Isotopic Effect in Double Proton Transfer Process of Porphycene Investigated by Enhanced QM/MM Method
Published on: July 19, 2019
Peculiarities of interaction of porphyrins with tRNA at low ionic strength
Y Dalyan1, I Vardanyan, A Chavushyan
1Yerevan State University, Faculty of Physics, Department of Molecular Physics, 1, A.Manoogian St., 0025, Yerevan, Armenia. yeva@ysu.am
Abstract:
The interaction of meso-tetra-(4N-oxyethylpyridyl)porphyrin (TOEPyP4) and its Zn(II)-, Cu(II)-, Mn(III)-derivatives with tRNA from E.Coli at low ionic strength (micro=0.02M) was studied using UV/Vis spectrophotometry and Circular Dichroism (CD) methods. An unusual Induced Circular Dichroism (ICD) spectra profile of the ZnTOEPyP4-tRNA complex is found. It is demonstrated that ZnTOEPyP4 is ordered in a stack, not only on helical sites, but also on loops of a hairpin form of tRNA. TOEPyP4 and CuTOEPyP4 are able to intercalate in the helical sites of this form of tRNA. MnTOEPyP4 interacts with tRNA via external non-ordered mechanism. It is established that all porphyrins are bound with tRNA more strongly than with DNA.
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