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Generation of Patient-Derived Podocytes from Skin Biopsies
Published on: May 26, 2023
Urinary biomarkers of podocyte injury
1Internal Medicine Department, Via Roma n. 67, Pisa University, 56100 Pisa, Italy. m.camici@med.unipi.it
Abstract:
Podocyturia may be an expression of glomerular disease and is evaluated using urinary podocyte-specific molecules. This article discusses the technical problems of detection and quantification of podocyturia using immunofluorescent staining of specific proteins, urinary podocyte culture, flow cytometry and urinary podocyte-specific molecules (including PCR, immunoblotting and ELISA). Technical problems mean that it is still impossible to define a biomarker for urinary podocyte injury. This article also discusses the future perspectives for podocyturia studies, including fluorescence-activated cell sorting and the study of the proteome of urinary exosomes.
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