Pregnane alkaloids from Pachysandra axillaris
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Updated: Jun 13, 2026

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Yun Sun1, Yu-Xin Yan, Jian-Chao Chen
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, People's Republic of China.
Abstract:
Nine new pregnane alkaloids, pachysamines J-R (1-9), together with seven known ones, were isolated from Pachysandra axillaris. The chemical structures of the new alkaloids were elucidated by spectroscopic methods. All the compounds were evaluated for their inhibitory activities against HL-60, SMMC-7721, A-549, SK-BR-3, and PANC-1 cell lines. Compound 15 possessed moderate activities against A-549, SK-BR-3, and PANC-1 cells, with the IC(50) values of 11.17, 4.17, and 10.76 microM, respectively. Besides, compound 11 showed cytotoxicities against A-549 cell, with the IC(50) values as 24.94 microM.
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