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Related Concept Videos

Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Signal Transduction: Overview01:26

Signal Transduction: Overview

Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
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Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Target Cell Response to Hormones01:22

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...

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Related Experiment Video

Updated: Jun 13, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Crosstalk and DC-SCRIPT: expanding nuclear receptor modulation.

M Ansems1, S Hontelez, N Karthaus

  • 1Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

Biochimica Et Biophysica Acta
|May 19, 2010
PubMed
Summary
This summary is machine-generated.

Nuclear receptors (NRs) regulate cell functions; their malfunction causes diseases. This review explores NR crosstalk and DC-SCRIPT/ZNF366 in balancing NR activity, focusing on breast cancer.

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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Last Updated: Jun 13, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Endocrinology

Background:

  • Nuclear receptors (NRs) are intracellular proteins that regulate gene transcription in response to hormones, vitamins, and metabolic products.
  • NRs control crucial physiological processes like growth, metabolism, and immunity, with dysregulation linked to diseases such as diabetes, inflammation, and cancer.
  • Cells express multiple NRs, leading to complex interactions with various ligands and potential crosstalk between receptor pathways.

Purpose of the Study:

  • To review novel insights into balancing nuclear receptor activity through NR crosstalk.
  • To discuss the role of DC-SCRIPT/ZNF366, a bi-functional NR coregulator, in modulating NR function.
  • To examine the impact of these regulatory mechanisms on breast cancer development and prognosis.

Main Methods:

  • Literature review focusing on recent advancements in nuclear receptor signaling.
  • Analysis of studies investigating nuclear receptor crosstalk and coregulator functions.
  • Synthesis of findings related to breast cancer pathogenesis and therapeutic strategies targeting NRs.

Main Results:

  • NR activity is finely tuned by complex crosstalk between different nuclear receptors.
  • DC-SCRIPT/ZNF366 acts as a critical bi-functional coregulator, influencing multiple NR pathways.
  • Dysregulation of NR crosstalk and coregulator function contributes to breast cancer progression.

Conclusions:

  • Understanding NR crosstalk and the role of coregulators like DC-SCRIPT/ZNF366 is crucial for deciphering complex cellular responses.
  • Targeting NR crosstalk and specific coregulators offers potential therapeutic avenues for breast cancer.
  • Further research into these intricate regulatory networks will advance our knowledge of nuclear receptor function in health and disease.