Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tailoring dry-ageing and cooking to enhance technological quality and volatile fingerprints in beef striploin.

Meat science·2026

Same author

Inflammation markers are associated with small extracellular vesicle protein signatures in cows with <i>Mycoplasma bovis</i>.

Frontiers in veterinary science·2026

Same author

<i>Mycoplasma bovis</i> infection alters small extracellular vesicle cargo derived from bovine endometrial epithelial cells cultured in static bioreactors.

Frontiers in microbiology·2026

Same author

The impact of a minimally processed seaweed meal on the appearance of amino acids in the peripheral circulation in healthy adults: A pilot dietary intervention.

Clinical nutrition ESPEN·2026

Same author

Characterizing the Flavor of New Zealand Native Plants Using Consumer-Derived Attributes and Gas Chromatography-Mass Spectrometry.

Journal of food science·2026

Same author

Decoding brewing yeast signatures: a MALDI-TOF MS approach to strain identification and beer style correlation.

Journal of applied microbiology·2025

Same journal

On-filter fractionation by empFASP improves identification of membrane peptides in proteomic experiments.

Journal of proteomics·2026

Same journal

Transpulmonary proteomic gradient analysis in women with pulmonary arterial hypertension associated with systemic sclerosis.

Journal of proteomics·2026

Same journal

Multiomic insights into fungal polylactic acid degradation: Metabolic adaptation and hydrolytic mechanisms of Sporobolomyces pararoseus.

Journal of proteomics·2026

Same journal

Temporal proteomic analysis reveals a three-phase adaptation strategy in Phytophthora cinnamomi during salinity stress.

Journal of proteomics·2026

Same journal

Proteomic and phosphoproteomic profiles of time-dependent dynamic changes in LPS-induced macrophage polarization.

Journal of proteomics·2026

Same journal

From prediction to mechanism: Explainable AI uncovers plasma and CSF proteomic signatures of Alzheimer's disease.

Journal of proteomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

Developing the wool proteome.

Stefan Clerens¹, Charisa D Cornellison, Santanu Deb-Choudhury

¹Protein & Structure, AgResearch, Lincoln Research Centre, Lincoln, New Zealand. stefan.clerens@agresearch.co.nz

Journal of Proteomics

|May 19, 2010

Summary

This summary is machine-generated.

This study significantly advances wool proteome research by identifying 113 wool proteins and adding ovine-specific sequences to databases. This breakthrough improves our understanding of wool fiber protein chemistry and proteomics.

More Related Videos

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Area of Science:

Proteomics
Biochemistry
Textile Science

Background:

The wool proteome remains largely uncharacterized due to database limitations and technical challenges.
Understanding wool protein composition is crucial for correlating physical properties and assessing damage from environmental or processing factors.

Purpose of the Study:

To achieve unprecedented identification of the wool proteome.
To expand the ovine-specific protein sequence database for future research.

Main Methods:

Employed a strategy of comprehensive data acquisition using hyphenated mass spectrometry (MS) techniques.
Utilized iterative database searching, result consolidation (ProteinScape), and rigorous validation.
Included liquid chromatography-tandem mass spectrometry (LC-MS/MS), LC-MALDI, 2D-LC-MS/MS, and SDS-PAGE LC-MS.

Main Results:

Successfully identified 113 wool proteins.
Added 72 complete and 30 partial ovine-specific protein sequences to the database.
Overcame previous limitations in wool proteome coverage and identification.

Conclusions:

This research significantly enhances the identification and characterization capabilities for ovine wool proteins.
Facilitates future research in wool fiber protein chemistry and proteomics.
Provides a foundation for investigating wool properties and damage mechanisms at a proteomic level.