Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-state-fermentation

Area of Science:

• Biotechnology
• Enzyme Engineering
• Microbial Fermentation

Background:

• Xylanase is a crucial enzyme with diverse industrial applications.
• Optimizing microbial production is key to cost-effective enzyme supply.
• Aspergillus niger is a well-established microbial host for enzyme production.

Purpose of the Study:

• To optimize solid-state fermentation conditions for enhanced xylanase production by Aspergillus niger DFR-5.
• To determine the optimal extraction parameters for efficient recovery of xylanase.
• To achieve a significant fold increase in concentrated xylanase production.

Main Methods:

• Solid-state fermentation using various substrates (wheat bran, soybean cake) and conditions (moisture, temperature, time).
• Optimization of extraction parameters using a central composite rotatable design (CCRD).
• Enzyme activity assay to quantify xylanase production and recovery.

Main Results:

• Highest xylanase activity (2596 IU/gds) achieved with a 70:30 wheat bran:soybean cake ratio, 70% moisture, and 6 days incubation at 40°C.
• Optimal extraction using water (10 ml/gds) at 200 rpm for 60 min yielded maximum recovery (4465±52 IU/gds).
• Overall 5.4-fold increase in concentrated xylanase production was achieved through optimized medium and extraction.

Conclusions:

• The study successfully optimized solid-state fermentation and extraction for Aspergillus niger xylanase.
• The findings provide a robust protocol for high-yield xylanase production.
• This optimized process has significant potential for industrial enzyme manufacturing.