Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complementary DNA sequencing: expressed sequence tags and human genome project.

M D Adams1, J M Kelley, J D Gocayne

  • 1Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.

Science (New York, N.Y.)
|June 21, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Testing the sociocultural model of eating disorders: A cross-national analysis of income inequality, gender equality, and eating disorder rates.

Social science & medicine (1982)·2026
Same author

Late toxicity after peposertib-enhanced chemoradiation in rectal cancer patients managed with organ preservation.

Clinical and translational radiation oncology·2026
Same author

Numerical study of the effect of the relative mobilities of chemical components on the non-solvent induced phase separation process for membrane elaboration.

The Journal of chemical physics·2025
Same author

Desiccation-induced DNA damage facilitates drug resistance in <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2025
Same author

[Evaluation of the performance of the artificial intelligence - enabled snail identification system for recognition of <i>Oncomelania hupensis robertsoni</i> and <i>Tricula</i>].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2025
Same author

Dynamic Reprogramming of Stromal Pdgfra-expressing cells during WNT-Mediated Transformation of the Intestinal Epithelium.

bioRxiv : the preprint server for biology·2025

Researchers generated expressed sequence tags (ESTs) from human brain complementary DNA (cDNA) to accelerate gene discovery and genome mapping. This rapid cDNA characterization offers a cost-effective approach for identifying new human genes and genetic markers.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Expressed sequence tags (ESTs) are valuable tools for gene discovery and genome mapping.
  • Characterizing human brain complementary DNA (cDNA) is crucial for understanding gene function and regulation.

Purpose of the Study:

  • To generate expressed sequence tags (ESTs) from human brain cDNA through automated partial DNA sequencing.
  • To assess the utility of ESTs for identifying novel human genes, mapping the genome, and locating coding regions.

Main Methods:

  • Automated partial DNA sequencing of over 600 human brain cDNA clones.
  • Polymerase chain reaction (PCR) amplification for chromosomal mapping of selected ESTs.

Main Results:

Related Experiment Videos

  • Generated a collection of expressed sequence tags (ESTs) from human brain cDNA.
  • Identified 337 novel genes, with 48 showing similarity to genes in other organisms (e.g., yeast, Drosophila, mouse).
  • Mapped 46 ESTs to specific human chromosomes using PCR.

    • Conclusions:

    • Automated cDNA characterization via ESTs is a rapid and cost-effective method for human gene discovery.
    • This approach facilitates the creation of a comprehensive gene map and provides valuable genetic markers.
    • The generated ESTs serve as a significant resource for diverse biological research fields.