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Updated: Jun 13, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
Published on: June 2, 2018
Memories of lost enhancers
Ranjan Sen1, Rudolf Grosschedl
1Laboratory of Cellular and Molecular Biology, National Institute of Aging, Baltimore, Maryland 21224, USA. rs465z@nih.gov
Abstract:
Transcriptional enhancers are key determinants of developmentally regulated gene expression. Models of enhancer function must distinguish between analog or digital control of transcription, as well as their requirement to initiate or maintain transcriptional activity of a gene. In light of a recent study by Chong and colleagues (pp. 659-669) providing evidence of a transient requirement of an enhancer associated with the CD4 gene, we discuss possible mechanisms by which transcriptional memory can be propagated in the absence of enhancers.
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