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Updated: Jun 13, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Correction of reabsorption artifacts in fluorescence spectra by the pinhole method
Henning Kühn1, Klaus Petermann, Günter Huber
1Institut für Laser-Physik, Universität Hamburg, Luruper Chaussee 149, D-22761 Hamburg, Germany. HKuehn@physnet.uni-hamburg.de
Abstract:
Reabsorption of spontaneously emitted photons by optically active ions in a laser material can significantly affect the measured emission spectra. A measurement technique to suppress reabsorpion artifacts in fluorescence spectra is reported. A theoretical description of the method as well as experimental results are presented. In combination with another version of the pinhole method for the determination of reabsorption-free fluorescence lifetimes, this technique allows one to suppress the influence of reabsorption artifacts in emission cross sections calculated by the Füchtbauer-Ladenburg equation.
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