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Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines
Ayako Kobori1, Shigeki Bamba, Hirotsugu Imaeda
1Division of Gastroenterology, Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu, Shiga, 520-2192, Japan.
Aim:
To investigate the effects of butyrate on interleukin (IL)-32alpha expression in epithelial cell lines.
Methods:
The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32alpha was determined by Western blotting analyses. IL-32alpha mRNA expression was analyzed by real-time polymerase chain reaction.
Results:
Acetate and propionate had no effects on IL-32alpha mRNA expression. Butyrate significantly enhanced IL-32alpha expression in all cell lines. Butyrate also up-regulated IL-1beta-induced IL-32alpha mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32alpha expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1beta-induced IL-32alpha mRNA expression.
Conclusion:
Butyrate stimulated IL-32alpha expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32alpha expression.
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