Related Experiment Video
Updated: Jun 12, 2026
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
Measurement of sVEGF R1 and PlGF in serum: comparing prototype assays from Beckman Coulter, Inc. to R&D Systems
Donald Wothe1, Emanuel Gaziano, Shiraz Sunderji
1Abbott Northwestern Hospital, Minneapolis, MN, USA.
Objective:
To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems.
Methods:
Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions.
Results:
Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF.
Conclusion:
There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.
