Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Magnetomicrometry.

Science robotics·2021

Same author

On prosthetic control: A regenerative agonist-antagonist myoneural interface.

Science robotics·2020

Same author

Tonic-clonic seizure after unrecognized unintentional dural puncture.

International journal of obstetric anesthesia·2020

Same author

Issues in using whole slide imaging for diagnostic pathology: "routine" stains, immunohistochemistry and predictive markers.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2013

Same author

Treatment of ulcerated necrobiosis lipoidica with ovine forestomach matrix.

Journal of wound care·2013

Same author

Energy benchmarks for water clusters and ice structures from an embedded many-body expansion.

The Journal of chemical physics·2013

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering of a Human 3D in vitro Tumor Test System

Published on: August 6, 2013

Tissue engineered tumor models.

M Ingram¹, G B Techy, B R Ward

¹Huntington Medical Research Institutes, 99 North El Molino Avenue, Pasadena, CA 91101-1830, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission

|May 21, 2010

Summary

This summary is machine-generated.

Researchers developed "tumor histoids," realistic 3-D models mimicking in vivo tumors with stromal and epithelial cells. These novel models offer improved in vitro tumor research and drug screening capabilities.

More Related Videos

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering of a Human 3D in vitro Tumor Test System

Published on: August 6, 2013

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

Area of Science:

Oncology
Biotechnology
Tissue Engineering

Background:

Traditional in vitro tumor models using monolayer cell cultures do not accurately represent in vivo tumor complexity.
Three-dimensional (3-D) tumor spheroids offer improved in vivo relevance but lack stromal-epithelial interactions.
Current models fail to capture the crucial microenvironmental interactions influencing tumor growth and development.

Purpose of the Study:

To develop a reproducible method for generating advanced 3-D tumor models.
To create more realistic in vitro tumor models that incorporate both stromal and malignant epithelial cells.
To establish a novel platform for pre-clinical drug screening and tumor microenvironment studies.

Main Methods:

A novel bioreactor system was designed for the reproducible generation of tumor histoids.
Disposable culture chambers (10 ml and 64 ml) were utilized to produce numerous histoid particles.
Histological analysis was performed on histoids derived from various cancer types (breast, prostate, colon, pancreas, urinary bladder).

Main Results:

A reproducible method for generating tissue-like 3-D tumor models, termed tumor histoids, was established.
The histoids closely mimic the architecture of in vivo tumor microlesions, containing both stromal and malignant epithelial cells.
The bioreactor system efficiently produces substantial quantities of histoid particles (100-600 per culture) with diameters in the tenths of millimeters.

Conclusions:

Tumor histoids represent a significant advancement in in vitro tumor modeling, offering enhanced realism over spheroids.
These models have broad applications, including pre-screening pharmaceuticals, serving as reference specimens for immunostaining, and studying tumor cell invasion.
The ability to sort histoids based on size and cell content using flow cytometry facilitates their use in drug testing and other research applications.