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Related Experiment Video

Updated: Jun 12, 2026

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

Microbial biofilms associated with biliary stent clogging.

Emilio Guaglianone¹, Rita Cardines, Claudia Vuotto

¹Department of Technologies and Health, Istituto Superiore di Sanità, Rome, Italy.

FEMS Immunology and Medical Microbiology

|May 21, 2010

Summary

This summary is machine-generated.

Microbial colonization, especially by anaerobic bacteria, contributes to biliary stent occlusion. This study identified various microbes and their biofilm formation, clarifying stent clogging mechanisms.

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Area of Science:

Gastroenterology and Hepatology
Microbiology
Biomaterials Science

Background:

Biliary obstruction often requires endoscopic stenting for palliation.
Stent occlusion is a major limitation, leading to cholangitis and requiring reintervention.
The role of microbial colonization, particularly anaerobic bacteria, in stent clogging is not fully understood.

Purpose of the Study:

To comprehensively identify aerobic and anaerobic bacteria and fungi colonizing explanted biliary stents.
To investigate the biofilm-forming capacity of isolated anaerobic bacterial strains.
To elucidate the mechanisms underlying biliary stent clogging.

Main Methods:

Analysis of 28 explanted biliary stents.
Microbial identification using culturing and denaturing gradient gel electrophoresis (DGGE).
Visualization of microbial colonization via scanning electron microscopy (SEM).

Main Results:

Identification of a diverse range of aerobic and anaerobic bacteria and fungi on stent surfaces.
Demonstration of biofilm formation by isolated anaerobic bacterial strains.
Evidence linking microbial colonization and biofilm development to stent occlusion.

Conclusions:

Microbial colonization, including anaerobic bacteria, is a significant factor in biliary stent clogging.
The ability of anaerobic bacteria to form biofilms contributes to stent occlusion.
Understanding these microbial mechanisms can inform strategies to prevent stent failure.