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Updated: Jun 12, 2026

Biophysical Characterization of Flagellar Motor Functions
Published on: January 18, 2017
Thermal and solvent-isotope effects on the flagellar rotary motor near zero load
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
Abstract:
In Escherichia coli, the behavior of the flagellar rotary motor near zero load can be studied by scattering light from nanogold spheres attached to proximal hooks of cells lacking flagellar filaments. We used this method to monitor changes in speed when cells were subjected to changes in temperature or shifted from a medium made with H(2)O to one made with D(2)O. In H(2)O, the speed increased with temperature in a near-exponential manner, with an activation enthalpy of 52 +/- 4 kJ/mol (12.0 +/- 1.0 kcal/mol). In D(2)O, the speed increased in a similar manner, with an activation enthalpy of 50 +/- 4 kJ/mol. The speed in H(2)O was higher than that in D(2)O by a factor of 1.53 +/- 0.14. We performed comparison studies of variations in temperature and solvent isotope, using motors operating at high loads. The variations were small, consistent with previous observations. The implications of these results for proton translocation are discussed.
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