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[Functional activity of thrombocytes and granulocytes of donor blood after selective removal of fibronectin]
Storage of anticoagulated whole blood leads to the activation of platelets and granulocytes, formation of aggregates, lysis of cells, various thrombogenic substances, including proteolytic enzymes. Transfusion of the blood may cause capillary occlusion, intravascular coagulation, renal and hepatic dysfunction. Gel-silicagel affinity+ chromatography of the anticoagulated whole blood was shown to result in selective sorption of plasma fibrin. Preactivated platelets and granulocytes were also removed by interaction of cell fibronectin receptors with immobilized fibronectin. The residual platelets and granulocytes lost their ability to storage-induced activation and remained viable for at least 48 hours at 4 degrees C.
Storage of anticoagulated whole blood leads to the activation of platelets and granulocytes, formation of aggregates, lysis of cells, various thrombogenic substances, including proteolytic enzymes. Transfusion of the blood may cause capillary occlusion, intravascular coagulation, renal and hepatic dysfunction. Gel-silicagel affinity+ chromatography of the anticoagulated whole blood was shown to result in selective sorption of plasma fibrin. Preactivated platelets and granulocytes were also removed by interaction of cell fibronectin receptors with immobilized fibronectin. The residual platelets and granulocytes lost their ability to storage-induced activation and remained viable for at least 48 hours at 4 degrees C.