[Dye analytical investigations on the polarization microscopy detection of collagen with Sominrot 4B (Part II)]

We use the disazo dyestuff Solaminrot 4B for the visualization of collagen fibres in the morphological substratum investigated. The dye selectively enhance the birefringency of the fibres. Therefore, the parts of this paper are dealing with the dye properties of 2 charges of Solaminrot 4B produced by Chemie AG Bitterfeld--Wolfen. The 1st charge contain supplementary substances; the 2nd charge is without ones. We used various physicochemical methods, e.g. chromatography, spectroscopy, and electrophoresis. Moreover, we analyzed the solubility of dye in inorganic and organic solventia, the phase dispersion in benzen and octanol, the influence of the dye probes on the pH values of the dye solutions, and so on. The results indicate that the physicochemical properties of both charges investigated by us are partially different. This is important for the application of Sirius Red dyestuffs to the histological staining procedures.