Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Complexometric Titration: Overview

Complexometric Titration: Overview

Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...

Redox Titration: Other Oxidizing and Reducing Agents

Redox Titration: Other Oxidizing and Reducing Agents

Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...

Complexometric Titration: Ligands

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of Dietary Sweet Potato Tuber Meal on Production Performance, Meat Quality and Intestine of Wenchang Chickens.

Biology·2026

Same author

Lanthanide Complexes-Based Probe for Dual-Modal Time-Gated Luminescence and Magnetic Resonance Imaging of Physiological pH Fluctuations.

Analytical chemistry·2026

Same author

An in-situ photoderivative fluorescence enhancement method without chromatographic separation for Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) detection.

Journal of hazardous materials·2026

Same author

A novel time-gated luminescent ruthenium(II) complex probe for highly sensitive detection of methylglyoxal in foods and complex biological samples.

Talanta·2026

Same author

Lanthanide complex-based dual-modal probes for background-free time-gated luminescence and <sup>19</sup>F magnetic resonance detection of hypochlorous acid in acute kidney injury.

The Analyst·2026

Same author

Lanthanide complex-based probes for time-gated luminescence and <sup>19</sup>F magnetic resonance imaging of hypochlorous acid in the liver.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

A lanthanide-complex-based ratiometric luminescent probe specific for peroxynitrite.

Cuihong Song¹, Zhiqiang Ye, Guilan Wang

¹Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|May 21, 2010

Summary

This summary is machine-generated.

A new ratiometric luminescence probe using lanthanide complexes can specifically detect peroxynitrite (ONOO(-)). This probe is effective for sensitive bioimaging in living cells.

More Related Videos

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Published on: October 22, 2012

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Related Experiment Videos

Last Updated: Jun 12, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Published on: October 22, 2012

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Area of Science:

Analytical Chemistry
Inorganic Chemistry
Biochemistry

Background:

Peroxynitrite (ONOO(-)) is a reactive nitrogen species implicated in various physiological and pathological processes.
Accurate detection of ONOO(-) is crucial for understanding its biological roles.
Existing detection methods may lack specificity or sensitivity for biological applications.

Purpose of the Study:

To design and synthesize a novel lanthanide-complex-based ratiometric luminescence probe for specific peroxynitrite detection.
To evaluate the probe's properties, including water solubility, stability, luminescence, and specificity towards ONOO(-).
To assess the probe's applicability for luminescence imaging detection of ONOO(-) in living cells.

Main Methods:

Synthesis of lanthanide complexes: [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)].
Characterization of luminescence properties (quantum yield, lifetime) and stability constants.
Testing the probe's response to ONOO(-) in aqueous buffers and its application in time-gated luminescence detection.
Investigating the probe's performance for luminescence imaging in living cells.

Main Results:

The synthesized [Eu(3+)/Tb(3+)(DTTA)] probe exhibits high water solubility and stability.
[Tb(3+)(DTTA)] luminescence is specifically quenched by ONOO(-), while [Eu(3+)(DTTA)] remains unaffected.
A ratiometric probe was successfully developed by mixing [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] for ONOO(-) detection.
The probe demonstrated high sensitivity and efficacy for ONOO(-) luminescence bioimaging in living cells.

Conclusions:

A novel, water-soluble, and stable ratiometric luminescence probe for peroxynitrite has been developed.
The probe offers specific and sensitive detection of ONOO(-) via time-gated luminescence.
The findings highlight the potential of this probe for advanced luminescence bioimaging applications in biological research.