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Published on: July 3, 2013
Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B(6)-deficient diet
Mustafa Keles1, Behcet Al, Kenan Gumustekin
1Department of Internal Medicine, Division of Nephrology, School of Medicine, Ataturk University, Erzurum, Turkey.
Introduction:
The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B(6)-deficient rats.
Material And Methods:
The rats were divided into control and vitamin B(6)-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mg/kg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer.
Results:
Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B(6)-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05).
Discussion:
The results show that vitamin B(6) deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats.
