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Related Experiment Videos

A "slow" temperature jump apparatus built from a stopped-flow machine.

W H Goldmann1, M A Geeves

  • 1University of Bristol, Department of Biochemistry, United Kingdom.

Analytical Biochemistry
|January 1, 1991
PubMed
Summary

A modified stopped-flow machine enables rapid temperature jumps for kinetic studies. This new temperature jump apparatus achieves >10°C changes in <150 ms, expanding accessible reaction time ranges.

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Area of Science:

  • Physical Chemistry
  • Chemical Kinetics
  • Biophysical Chemistry

Background:

  • Standard stopped-flow machines are primarily for rapid mixing, not temperature control.
  • Conventional temperature jump methods have limitations in achievable temperature change and response time.

Purpose of the Study:

  • To describe a simple modification of a thermostated stopped-flow machine.
  • To adapt a stopped-flow apparatus for use as a temperature jump machine.

Main Methods:

  • Modification of a standard thermostated stopped-flow instrument.
  • Utilizing the modified apparatus to induce temperature jumps in a sample.

Main Results:

  • Achieved temperature jumps greater than 10 degrees C.

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  • Temperature jump response time of less than 150 milliseconds.
  • Apparatus accommodates a 300 microliter sample volume.
  • Capable of both heating and cooling jumps relative to initial temperature.

    • Conclusions:

    • The modified stopped-flow machine functions effectively as a temperature jump apparatus.
    • This method is suitable for studying reactions within a time range not covered by conventional techniques.
    • The apparatus offers flexibility with adjustable jump magnitudes and directions.