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Effect of Pro-Leu-Gly-NH(2) on plasma levels of ?-MSH in the rat
M E Celis1, A J Thody, C Fisher
1Wellcome Laboratories for Research into Skin Diseases, Department of Dermatology, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K.
Abstract:
With the idea to give further support that Pro-Leu-Gly-NH(2) (PLG) acts as MIF (the inhibitor factor of MSH), this paper reports the effect of PLG on the secretion of MSH release using a recently developed radioimmunoassay for ?-MSH. Pro-Leu-Gly-NH(2) was effective in inhibiting the release of MSH induced by the injection of acid extracts of median eminence (MRF). The rise in plasma MSH by these extracts was not due to their intrinsic content of MSH. Pro-Leu-Gly-NH(2) also inhibited the basal secretion of MSH in male rats. Since PLG also blocked the release of MSH induced by the injection of haloperidol, it is suggested that its effect is not mediated by dopamine.
Insights
Pro-Leu-Gly-NH(2) (PLG) inhibits the release of melanocyte-stimulating hormone (MSH) in rats. This peptide acts as a MIF (MSH release inhibiting factor), independent of dopamine pathways.
Area of Science:
- Neuroendocrinology
- Peptide Hormone Research
Background:
- Melanocyte-stimulating hormone (MSH) plays a role in various physiological processes.
- The existence and function of MSH release inhibiting factor (MIF) are crucial for regulating MSH secretion.
Purpose of the Study:
- To investigate the inhibitory effect of Pro-Leu-Gly-NH(2) (PLG) on MSH release.
- To provide further evidence for PLG acting as a MIF.
Main Methods:
- Utilized a newly developed radioimmunoassay for ?-MSH to quantify MSH levels.
- Administered PLG to male rats and measured MSH release under different conditions (basal, induced by median eminence extracts, induced by haloperidol).
Main Results:
- PLG effectively inhibited MSH release induced by acid extracts of the median eminence.
- The observed increase in plasma MSH from these extracts was not due to their inherent MSH content.
- PLG also suppressed basal MSH secretion in male rats.
- PLG inhibited MSH release induced by haloperidol, suggesting a non-dopaminergic mechanism.
Conclusions:
- Pro-Leu-Gly-NH(2) (PLG) functions as a MSH release inhibiting factor (MIF).
- The inhibitory action of PLG on MSH release is not mediated through the dopamine system.
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