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Related Concept Videos

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jun 12, 2026

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
12:06

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex

Published on: August 19, 2025

Synaptosomal cytoskeleton visualized by whole mount electron microscopy.

T Suzuki1, T Fujii, R Tanaka

  • 1Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467, Japan.

Neurochemistry International
|May 22, 2010
PubMed
Summary
This summary is machine-generated.

Researchers visualized synaptosome cytoskeleton organization using whole mount electron microscopy. They identified distinct pre- and post-synaptic structures, revealing actin

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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
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Related Experiment Videos

Last Updated: Jun 12, 2026

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
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Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex

Published on: August 19, 2025

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
12:14

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Published on: September 14, 2022

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Synaptosomes are crucial for neuronal communication.
  • Understanding their cytoskeletal organization is key to synaptic function.

Purpose of the Study:

  • To visualize and characterize the synaptosomal cytoskeleton.
  • To identify distinct pre- and post-synaptic cytoskeletal components.

Main Methods:

  • Whole mount electron microscopy of rat cerebrum synaptosomes.
  • Partial solubilization using various detergents.
  • Chemical treatments to reveal cytoskeletal structures.

Main Results:

  • Identified three types of synaptosomal cytoskeletons: pre-synaptic fiber networks (15-20 nm fibers, 60-100 nm rings), post-synaptic fiber aggregates (10 nm filaments, >95% of Triton-resistant cytoskeleton), and potential artifactual connecting fibers.
  • Pre-synaptic networks differed from mitochondrial structures.
  • Actin was detected in both pre- and post-synaptic cytoplasm.

Conclusions:

  • Synaptosomes possess complex, distinct pre- and post-synaptic cytoskeletal organizations.
  • The post-synaptic structure is a major component of the synaptic plasma membrane cytoskeleton.
  • Actin plays a role in both pre- and post-synaptic compartments.