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Updated: Jun 12, 2026

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
Published on: December 30, 2017
On the disposition of phospholipids in freshly isolated myelin sheath preparations from bovine brain
K Gwarsha1, M G Rumsby, C Little
1Department of Biology, University of York, Heslington, York Y01 5DD, U.K.
Abstract:
The extent of availability of the four major classes of phospholipids in freshly isolated bovine brain myelin has been studied using phospholipase C (Clostridium perfringens), phospholipase A (bee venom), sphingomyelase (Staphalococcus aureus) and trinitrobenzenesulphonate. The results have been compared with those of a previous study (Gwarsha et al., 1984). Taken together, the data suggest that 47-67% of the phosphatidylcholine, 45-66% of the ethanolamine-containing phosphoglycerides, 52-55% of the spingomyelin and 10-32% of the phosphatidylserine + phosphatidylinositol in the fresh myelin is available to these probes and hence may be located at the external surface of the membrane bilayer.
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