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Common genetic polymorphisms in pre-microRNAs were associated with increased risk of dilated cardiomyopathy

Bin Zhou1, Li Rao, Ying Peng

  • 1Laboratory of Molecular Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, PR China.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|May 22, 2010
PubMed
Abstract

Insights

Common single nucleotide polymorphisms in pre-microRNAs are linked to increased risk of dilated cardiomyopathy (DCM). Specifically, variants in has-mir-196a2 and hsa-mir-499 are associated with higher DCM risk.

Area of Science:

  • Genetics
  • Molecular Biology
  • Cardiology

Background:

  • Single nucleotide polymorphisms (SNPs) in pre-microRNAs can alter microRNA (miRNA) expression and function.
  • These alterations may lead to various functional consequences, potentially impacting disease development.

Purpose of the Study:

  • To investigate the association between common SNPs in pre-microRNAs and the risk of developing dilated cardiomyopathy (DCM).
  • This pilot study aimed to identify specific pre-miRNA SNPs linked to DCM susceptibility.

Main Methods:

  • Genotyping of three pre-miRNA SNPs (has-mir-196a2 rs11614913 C/T, hsa-mir-499 rs3746444 A/G, hsa-mir-146a rs2910164 C/G) was performed.
  • The study included 221 DCM patients and 321 healthy control subjects, with genotyping conducted using PCR-restriction fragment length polymorphism (RFLP) assay.

Main Results:

  • The variant alleles of has-mir-196a2 rs11614913 (T allele) and hsa-mir-499 rs3746444 (G allele) were significantly associated with increased DCM risk (P<0.0001 for both).
  • A dominant model showed a statistically significant association between these two SNPs and increased DCM risk.
  • No significant association was observed between DCM risk and the hsa-mir-146a rs2910164 C/G polymorphism (P=0.451).

Conclusions:

  • The study concludes that common genetic polymorphisms in pre-microRNAs, specifically has-mir-196a2 rs11614913 C/T and hsa-mir-499 rs3746444 A/G, are associated with a significantly increased risk of dilated cardiomyopathy.
  • The hsa-mir-146a rs2910164 C/G polymorphism was not found to be associated with DCM risk in this cohort.

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