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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
Published on: February 27, 2020
The archaeal exosome localizes to the membrane
Verena Roppelt1, Cédric F V Hobel, Sonja V Albers
1Institut für Mikrobiologie und Molekularbiologie der JLU Giessen, Giessen, Germany.
FEBS Letters
|May 22, 2010
Summary
The archaeal exosome, an RNA-processing complex, is membrane-bound in Sulfolobus solfataricus. This suggests spatial organization of RNA processing in Archaea.
Area of Science:
- Cellular Biology
- Molecular Biology
- Archaea Research
Background:
- The archaeal exosome is an RNA-processing complex.
- It contains eukaryotic orthologs (Rrp41, Rrp42, Rrp4, Csl4) and an archaea-specific subunit (DnaG).
Purpose of the Study:
- To determine the cellular localization of the archaeal exosome.
- To investigate the spatial organization of RNA processing in Archaea.
Main Methods:
- Cell-free extract fractionation using sucrose density gradients.
- Immunofluorescence microscopy.
Main Results:
- The DnaG and Rrp41 subunits were enriched in a distinct ring fraction, co-localizing with surface layer proteins.
- Immunofluorescence confirmed DnaG and Rrp41 localization at the cell periphery.
- These findings implicate a membrane-bound state for the archaeal exosome.
Conclusions:
- The archaeal exosome is membrane-bound in Sulfolobus solfataricus.
- RNA processing in Archaea appears to be spatially organized.
- This localization may be linked to surface layer proteins.
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