Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IGFD-Net: Illumination-guided frequency decoupling for polarization image fusion.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Cost Effectiveness of Transrectal and Transperineal Prostate biopsy: Analysis from the ProBE-PC Randomized Study.

Urology practice·2026
Same author

CDK8 and CDK19 Mediator kinases are required for hepatitis delta virus replication.

Hepatology (Baltimore, Md.)·2026
Same author

Host-derived interleukin-1α drives tumor immunosuppression by reprogramming tumor-associated myeloid cells.

NPJ breast cancer·2026
Same author

Inhibition of CDK8 rescues impaired ischemic fracture healing.

NPJ Regenerative medicine·2026
Same author

Anoikis resistance and metastasis of ovarian cancer can be overcome by CDK8/19 mediator kinase inhibition.

JCI insight·2026

Related Experiment Video

Updated: Jun 12, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Gli2 expression and human bladder transitional carcinoma cell invasiveness.

Clay W Mechlin1, Matthew J Tanner, Mengqian Chen

  • 1Division of Urology, Albany Medical College, Albany, New York, USA. mianb@mail.amc.edu

The Journal of Urology
|May 22, 2010
PubMed
Summary

Gli2 expression, not hedgehog signaling, drives bladder cancer cell invasiveness. This study reveals Gli2

More Related Videos

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
11:02

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 12, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
11:02

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Hedgehog signaling pathway regulates Gli transcription factors.
  • Aberrant hedgehog signaling is implicated in oncogenesis.
  • Targeting hedgehog signaling is a strategy for anticancer drug development.

Purpose of the Study:

  • To investigate the role of hedgehog/Gli signaling in human bladder transitional cell carcinoma (TCC).
  • To determine the involvement of hedgehog/Gli signaling in the proliferative and invasive behaviors of bladder TCC cells.

Main Methods:

  • Stratified human bladder TCC cell lines based on growth rate and invasiveness.
  • Assessed growth inhibition using hedgehog pathway inhibitors (cyclopamine) and inactive controls (tomatidine).
  • Quantified expression of hedgehog signaling components via RT-PCR.
  • Manipulated Gli2 expression and activity to evaluate effects on proliferation and invasiveness.

Main Results:

  • Cell invasiveness and growth rates were stratified across tested bladder TCC lines.
  • Weak growth inhibition observed with tomatidine and cyclopamine across all cell lines.
  • Gli2 expression levels correlated with cell invasiveness, unlike other hedgehog pathway components.
  • Modulating Gli2 expression or activity significantly impacted cell invasiveness.

Conclusions:

  • Hedgehog signaling does not appear to drive bladder cancer cell proliferation.
  • Gli2 expression is strongly associated with bladder cancer cell invasive behavior.
  • Gli2, potentially through noncanonical signaling, contributes to bladder cancer invasiveness.