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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 12, 2026

A Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings
08:23

A Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings

Published on: September 30, 2019

Magnified grating images used in displacement sensing.

K Hane, C P Grover

    Applied Optics
    |May 22, 2010
    PubMed
    Summary

    Researchers achieved highly magnified images of periodic objects near a rectangular grating using incoherent illumination. This optical transfer function analysis enables precise displacement measurements and explains Lau imaging phenomena.

    Area of Science:

    • Optics and Photonics
    • Image Science

    Background:

    • Periodic objects and gratings are fundamental in optical systems.
    • Understanding imaging behavior is crucial for metrology and advanced imaging techniques.

    Purpose of the Study:

    • To obtain and analyze highly magnified images of periodic objects near a rectangular grating.
    • To quantitatively explain the imaging behavior using optical transfer function (OTF).
    • To explore applications in precise displacement measurements and explain Lau imaging.

    Main Methods:

    • Incoherent illumination of a periodic object near a rectangular grating.
    • Analysis of imaging characteristics via the system's optical transfer function.
    • High magnification imaging techniques.

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    Published on: July 5, 2016

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    Last Updated: Jun 12, 2026

    A Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings
    08:23

    A Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings

    Published on: September 30, 2019

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    Main Results:

    • Successful acquisition of highly magnified images.
    • Quantitative explanation of imaging behavior based on OTF.
    • Demonstration of the phenomenon's utility for precise displacement measurements.

    Conclusions:

    • The study provides a quantitative understanding of imaging periodic objects near gratings.
    • High magnification imaging offers a viable method for precise displacement metrology.
    • The phenomenon is a spatial case related to Lau imaging principles.