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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 12, 2026

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues
10:18

High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

Published on: September 14, 2016

Computerized extraction of the time dimension in histopathological sections.

G Zajicek, E Bartfeld, D Schwartz-Arad

    Applied Optics
    |May 22, 2010
    PubMed
    Summary

    Cell age in renewing tissues can be measured by distance from the origin. This study shows hepatocyte DNA content increases with cell age, correlating with optical density.

    Area of Science:

    • Cell biology
    • Tissue renewal dynamics
    • Biophysics

    Background:

    • Most organism tissues continuously renew cells via a two-compartment system: progenitor (P) and functional (Q) compartments.
    • Cells move outward along a tissue radius, ordered by age, with older cells located further from the origin.
    • This radial movement reflects a continuous cell stream, where each cell's position indicates its age and history.

    Purpose of the Study:

    • To investigate the relationship between cell age and DNA content in renewing tissues.
    • To validate the concept of using radial distance as a proxy for cell age.
    • To demonstrate a method for quantifying cell age and its biological correlates.

    Main Methods:

    • Developing a tissue analyzer software to sample cells along the tissue radius.

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  • Utilizing Feulgen staining to label DNA in hepatocytes.
  • Measuring the optical density of stained hepatocytes to infer DNA content.

    • Main Results:

    • Cellular age is directly proportional to its distance from the origin within the tissue radius.
    • Hepatocyte optical density, indicative of DNA content, increases with cell age.
    • Feulgen staining binds stoichiometrically to DNA, confirming DNA content increases with hepatocyte age.

    Conclusions:

    • Cellular age in renewing tissues can be accurately determined by measuring radial distance.
    • Hepatocyte DNA content is a reliable biomarker for cell age.
    • The developed methodology provides a quantitative approach to study cell aging and renewal processes.