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Related Concept Videos

Light Acquisition02:16

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jun 12, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Real-time joint transform correlation by partially coherent readout illumination.

B Javidi

    Applied Optics
    |May 22, 2010
    PubMed
    Summary

    This study introduces a real-time joint Fourier transform image correlator using partially coherent light. This method reduces phase distortion, improving correlation peak intensity and signal-to-noise ratio for pattern recognition.

    Area of Science:

    • Optics and Photonics
    • Image Processing
    • Real-time Optical Correlators

    Background:

    • Traditional joint Fourier transform correlators (JFTCs) can suffer from performance degradation due to spatial phase variations from spatial light modulators (SLMs).
    • These phase distortions arise from non-uniformities in SLM output wavefronts, impacting correlator accuracy.

    Purpose of the Study:

    • To develop a real-time joint Fourier transform image correlator that mitigates spatial phase variations.
    • To enhance signal detection in complex amplitude by utilizing partially coherent readout illumination.

    Main Methods:

    • Implementation of a real-time JFTC employing partially coherent readout illumination for signal detection.
    • Interference of Fourier transforms using coherent light, with correlation signal generated by partially coherent illumination of the SLM output.

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  • Experimental validation using naturally illuminated real objects for pattern recognition.
  • Main Results:

    • Demonstrated reduction of phase distortion effects from the SLM output surface using partially coherent readout illumination.
    • Observed a smaller correlation bandwidth compared to VanderLugt filtering under quasi-monochromatic illumination.
    • Achieved brighter correlation peak intensity and improved signal-to-noise ratio (SNR).

    Conclusions:

    • Partially coherent readout illumination effectively reduces phase distortion in real-time JFTCs.
    • The proposed technique enhances correlation performance, leading to brighter peaks and better SNR.
    • This method offers a viable approach for robust pattern recognition with improved optical correlator systems.