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Primary structure of a trisialylated oligosaccharide from human milk
S Fievre1, J M Wieruszeski, J C Michalski
1Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS No 111, Villeneuve d'Ascq, France.
Biochemical and Biophysical Research Communications
|June 14, 1991
Abstract:
A novel oligosaccharide has been isolated from human milk and identified as a trisialylated derivative of lacto-N-hexaose. This compound was purified by combining ion-exchange chromatography, preparative paper chromatography and high performance liquid chromatography. Structural analyses by methylation, fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy are consistent with the following structure: formula: see text.