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Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
Published on: January 15, 2018
[Recombinant adenovirus mediate RNA silencing of perforin in NK-92 cells and its function]
1First Affiliated Hospital, Chongqing Medical University, Chongqing, China. zhaolilin263@163.com
Aim:
To adenovirus-mediated RNAi technology be used to down-regulate perforin expression in NK-92 cells. This study will provide convenience for further investigation of the mechanism of perforin.
Methods:
First, we designed three perforin shRNAs (shPF) and identified their knockdown efficiency at mRNA levels. Then we constructed recombinant adenovirus with shPF. After that, we transfected NK-92 cells with Ad-shPF and detected the expression of perforin at mRNA and protein levels.
Results:
We confirm that the design of shPF sequences are effective. Moreover, the mRNA level of NK-92 cells treated by Ad-shPFP1 drop to 32% compare to Ad-scramble treated control. Furthermore, using Western blot, we find Ad-shPF1 treatment can obviously down-regulate the expression of perforin protein.
Conclusion:
Recombinant adenovirus with perforin shRNA can knockdown the expression of perforin in NK-92 cells.
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