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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

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Updated: Jun 12, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Hyphenations in planar chromatography.

Gertrud Morlock1, Wolfgang Schwack

  • 1University of Hohenheim, Institute of Food Chemistry, Stuttgart, Germany. gmorlock@uni-hohenheim.de

Journal of Chromatography. A
|May 25, 2010
PubMed
Summary
This summary is machine-generated.

High-performance thin-layer chromatography (HPTLC) offers versatile hyphenation capabilities for efficient analysis. This planar chromatography technique enhances natural product, food, and lipid analysis through hyphenation with mass spectrometry and effect-directed analysis.

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Last Updated: Jun 12, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

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Published on: September 21, 2011

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Planar chromatography, particularly high-performance thin-layer chromatography (HPTLC), is a key technique.
  • The open, planar stationary phase allows for hyphenation and post-chromatographic evaporation.

Purpose of the Study:

  • To review the applications and advantages of HPTLC, focusing on hyphenation.
  • To demonstrate the efficiency gains in various analytical fields.

Main Methods:

  • Review of existing literature on HPTLC and its hyphenated techniques.
  • Discussion of hyphenation with effect-directed analysis (EDA) and mass spectrometry (MS).

Main Results:

  • HPTLC facilitates hyphenation with EDA and MS for enhanced analytical workflows.
  • Demonstrated efficiency gains in natural product, food, and lipid analysis.
  • Parallel analysis of multiple samples is achievable.

Conclusions:

  • HPTLC's planar format provides unique flexibility for complex separation challenges.
  • Hyphenation strategies can be tailored to specific analytical needs, improving information retrieval.
  • The technique offers valuable assistance to separation scientists.