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Updated: Jun 12, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

RNA deep sequencing of the Atlantic cod transcriptome.

Steinar D Johansen¹, Bård Ove Karlsen, Tomasz Furmanek

¹Bodø University College, Norway. Steinar.Johansen@uit.no

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics

|May 25, 2010

Summary

Researchers are sequencing the Atlantic cod (Gadus morhua) transcriptome to understand gene expression across different life stages and tissues. This deep sequencing effort provides valuable genomic resources for this important aquaculture species.

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Novel Sequence Discovery by Subtractive Genomics

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Published on: January 25, 2019

Area of Science:

Aquaculture genomics
Marine biology
Transcriptomics

Background:

Atlantic cod (Gadus morhua) is a key species in marine aquaculture.
Genomic resources for Atlantic cod are under development.
Understanding gene expression is crucial for aquaculture development.

Purpose of the Study:

To characterize the Atlantic cod transcriptome using deep sequencing.
To analyze expressed genes across various developmental and tissue types.
To identify key RNA molecules, including mRNAs, intermediate RNAs, and microRNAs.

Main Methods:

Deep sequencing using Roche 454, Illumina GA, and ABI SOLiD platforms.
Bioinformatic analysis of sequencing data.
Investigation of both embryonic and adult tissues.

Main Results:

Comprehensive transcriptome data generated for Atlantic cod.
Analysis of nuclear and mitochondrial polyadenylated messenger RNAs (mRNAs).
Characterization of non-protein-coding intermediate RNAs and regulatory microRNAs.

Conclusions:

The study provides a valuable transcriptomic dataset for Atlantic cod.
These findings contribute to the genomic characterization of an emerging aquaculture species.
The identified RNA molecules offer insights into gene regulation and development in Atlantic cod.