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Updated: Jun 12, 2026

Assembly and Purification of Prototype Foamy Virus Intasomes
Published on: March 19, 2018
Protein binding sites involved in the assembly of the KplE1 prophage intasome
Gaël Panis1, Yohann Duverger, Stéphanie Champ
1Laboratoire de Chimie Bactérienne CNRS UPR9043, Institut de Microbiologie de la Méditerranée, Marseille Cedex 20, France.
Abstract:
The organization of the recombination regions of the KplE1 prophage in Escherichia coli K12 differs from that observed in the lambda prophage. Indeed, the binding sites characterized for the IntS integrase, the TorI recombination directionality factor (RDF) and the integration host factor (IHF) vary in number, spacing and orientation on the attL and attR regions. In this paper, we performed site-directed mutagenesis of the recombination sites to decipher if all sites are essential for the site-specific recombination reaction and how the KplE1 intasome is assembled. We also show that TorI and IntS form oligomers that are stabilized in the presence of their target DNA. Moreover, we found that IHF is the only nucleoid associated protein (NAP) involved in KplE1 recombination, although it is dispensable. This is consistent with the presence of only one functional IHF site on attR and none on attL.
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