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Related Experiment Video

Updated: Jun 12, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
07:07

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Published on: March 12, 2021

Comparative analysis on gene expression profiles in cattle subcutaneous fat tissues.

Masaaki Taniguchi1, Le Luo Guan, John A Basarab

  • 1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
|May 25, 2010
PubMed
Summary

Researchers identified genes linked to fat metabolism in beef cattle by analyzing differences in gene expression in subcutaneous adipose tissue. These findings offer insights into backfat development and potential genetic markers for improved meat value.

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Area of Science:

  • Animal Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Fat-related carcass traits significantly impact the economic value of beef.
  • Understanding fat metabolism is crucial for improving beef quality and industry value.

Purpose of the Study:

  • To identify genes associated with fat metabolism in beef cattle.
  • To investigate differentially expressed genes in subcutaneous adipose tissue related to backfat thickness.

Main Methods:

  • Microarray analysis using a bovine-specific oligo-platform (8329 probes).
  • Bioinformatic tools for functional characterization of differentially expressed genes (DEGs).
  • Quantitative real-time PCR (qPCR) for validation of selected DEGs.

Main Results:

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Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue
06:50

Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue

Published on: March 29, 2024

Related Experiment Videos

Last Updated: Jun 12, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
07:07

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Published on: March 12, 2021

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue
06:50

Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue

Published on: March 29, 2024

  • Identified 360 DEGs, with 45 involved in 82 KEGG pathways.
  • Confirmed correlations between backfat thickness and expression levels of 6 selected genes.
  • Observed genotype-specific differences in gene expression and fat metabolism mechanisms.

Conclusions:

  • Gene expression differences in novel and known genes are associated with backfat development in beef cattle.
  • Potential genetic markers for fat-related traits identified.
  • Fat metabolism mechanisms may vary across different cattle genotypes.