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Updated: Jun 12, 2026
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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Streptomyces aomiensis sp. nov., isolated from a soil sample using the membrane-filter method.
Aya Nagai1, Shams Tabrez Khan1, Tomohiko Tamura2
1Biomedicinal Information Research Center (BIRC), Japan Biological Informatics Consortium (JBIC), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Summary
A novel soil bacterium, Streptomyces aomiensis, was discovered in Japan. This Gram-positive actinobacterium represents a new species within the Streptomyces genus, expanding microbial diversity knowledge.
Area of Science:
- Microbiology
- Actinobacteria research
- Soil microbiology
Background:
- Actinobacteria, particularly the genus Streptomyces, are crucial soil inhabitants with significant biotechnological potential.
- The isolation and characterization of novel Streptomyces species contribute to understanding microbial diversity and discovering new bioactive compounds.
Purpose of the Study:
- To isolate and characterize a novel actinobacterium from a Japanese soil sample.
- To determine the taxonomic position of the isolated strain through polyphasic analysis.
- To propose a new species designation for the novel isolate.
Main Methods:
- Isolation of actinobacteria using the membrane-filter method from a soil sample.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Chemotaxonomic analyses including cell wall hydrolysates and isoprenoid quinones.
- Determination of genomic DNA G+C content.
Main Results:
- A distinct Gram-positive actinobacterium, strain M24DS4(T), was isolated from Aomi, Tokyo, Japan.
- The isolate showed low 16S rRNA gene sequence similarity (96.1%) to its closest relative, Streptomyces scabrisporus.
- Chemotaxonomic data revealed the presence of ll-diaminopimelic acid and MK-9(H(8))/MK-9(H(6)) quinones.
- The genomic DNA G+C content was determined to be 75 mol%.
Conclusions:
- Strain M24DS4(T) represents a novel species within the genus Streptomyces based on its distinct phylogenetic and chemotaxonomic characteristics.
- The proposed name for this new species is Streptomyces aomiensis sp. nov.
- The type strain is M24DS4(T) (=NBRC 106164(T) =KACC 14925(T)).
