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Increased oxidative stress in healthy children following an exercise program: a pilot study
Melita M Nasca1, Renliang Zhang, Dennis M Super
1Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Summary
Exercise significantly increased oxidative stress markers in children. This study measured urinary 8-isoprostaglandin F2alpha (8-iso-PGF2 alpha), a marker of lipid peroxidation, before and after a 5-week program.
Area of Science:
- Pediatric Exercise Science
- Biochemistry
- Sports Medicine
Background:
- Exercise can induce oxidative stress, an imbalance between reactive oxygen species and antioxidant defenses.
- Systemic oxidative stress is a key factor in health and disease.
Purpose of the Study:
- To investigate the effect of a real-life exercise program on systemic oxidative stress in children.
- To measure urinary 8-isoprostaglandin F2alpha (8-iso-PGF2 alpha) concentrations as a noninvasive index of lipid peroxidation.
Main Methods:
- A 5-week track and field summer camp program was conducted for 6 healthy, primarily sedentary children aged 8-10 years.
- Participants were categorized into healthy weight, overweight, and obese groups.
- Urinary 8-iso-PGF2 alpha concentrations were measured using high-performance liquid chromatography with online electrospray ionization tandem mass spectrometry (LC/ESI/MS/MS).
Main Results:
- A significant increase (p = .028) in mean urinary 8-iso-PGF2 alpha concentration was observed post-exercise compared to pre-exercise.
- This increase in oxidative stress marker was consistent at the individual level.
- Preliminary evidence suggested correlations between 8-iso-PGF2 alpha concentrations and children's cardiometabolic characteristics and mood.
Conclusions:
- The study indicates that exercise programs can increase oxidative stress markers in children.
- Further research is warranted to explore the relationship between exercise-induced oxidative stress and cardiometabolic health, lifestyle factors, and mood in pediatric populations.
- Understanding whether this increase is adaptive or indicative of injury is crucial for optimizing exercise interventions.
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Definition
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Purposes
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