Testosterone in MPOA elicits behavioral but not neuroendocrine responses in ferrets

The amount of time male ferrets were engaged in neck gripping, mounting, and thrusting was quantified in 30-min tests with a receptive female before and after castration. Bilateral cannulae containing a total of approximately 2 mg testosterone propionate (TP) in cocoa butter were then stereotaxically aimed at the medial preoptic area (MPOA). Tests for sexual behavior were conducted on days 3, 7, 14, and 21 postimplantation. Ferrets were histologically categorized as either 1) Miss (implants not in MPOA), 2) Unilateral implant in MPOA, or 3) Bilateral implants in MPOA. The mean amount of time spent neck gripping, mounting, and thrusting increased significantly over castrate levels on postimplantation day 14 in the Bilateral group, but not in the Miss or Unilateral groups. In all groups, mean plasma testosterone concentrations were below or near the lower limit of detectability on the day before TP implantation and on postimplantation test days. In the same plasma samples, luteinizing hormone concentrations were within the normal range of castrated ferrets, and did not significantly decline after TP implantation. These results suggest that the MPOA is a neural site for androgen activation of certain components of reproductive behavior but not for negative feedback on gonadotropin secretion in male ferrets.