Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pineal microdialysis in freely moving rats.

T Azekawa1, A Sano, H Sei

  • 1Department of Physiology, School of Medicine, University of Tokushima, Japan.

Brain Research Bulletin
|March 1, 1991
PubMed
Summary

A new surgical method allows for chronic in vivo microdialysis in rat pineal glands. This technique avoids sinus ligation and minimizes bleeding, preserving essential sympathetic innervation for pineal function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical characteristics and outcomes of pheochromocytoma crisis: a literature review of 200 cases.

Journal of endocrinological investigation·2022
Same author

Paraneoplastic pemphigus associated with Castleman disease: progression from mucous to mucocutaneous lesions with epitope-spreading phenomena.

The British journal of dermatology·2017
Same author

Preoperative prediction for regaining ambulatory ability in paretic non-ambulatory patients with metastatic spinal cord compression.

Spinal cord·2016
Same author

Paracoccidioides brasiliensis and P. lutzii antigens elicit different serum IgG responses in chronic paracoccidioidomycosis.

Mycopathologia·2013
Same author

Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats.

Neuroscience·2013
Same author

Epithelial to mesenchymal transition in murine tracheal allotransplantation: an immunohistochemical observation.

Transplantation proceedings·2013

Area of Science:

  • Neuroscience
  • Surgical Techniques
  • Physiology

Background:

  • The pineal gland's function is regulated by sympathetic innervation.
  • In vivo microdialysis is a valuable tool for studying neuroendocrine function.
  • Previous techniques for pineal gland cannulation posed challenges.

Purpose of the Study:

  • To describe a novel surgical technique for implanting guide cannulas in the rat pineal gland.
  • To enable chronic in vivo microdialysis in freely moving rats.
  • To preserve the integrity of the pineal gland's microenvironment and innervation.

Main Methods:

  • A refined surgical approach for guide cannula implantation in the rat pineal gland.
  • Technique avoids ligation of superior or transverse sinuses.

Related Experiment Videos

  • Minimizes bleeding from dural veins and preserves sympathetic nerve fibers.

    • Main Results:

    • Successful chronic implantation of guide cannulas in the rat pineal gland.
    • The technique demonstrated minimal bleeding and preservation of dural integrity.
    • Sympathetic innervation to the pineal gland remained undisturbed.

    Conclusions:

    • This surgical technique facilitates chronic in vivo microdialysis of the rat pineal gland.
    • The method is advantageous due to its minimally invasive nature and preservation of pineal gland function.
    • Enables advanced research into pineal gland physiology and neuroendocrine regulation.