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Updated: Jun 12, 2026

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
Published on: October 30, 2021
Identification of palmatine as an inhibitor of West Nile virus
Fan Jia1, Gang Zou, Jingjing Fan
1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Abstract:
In this study, we investigated the specific inhibition of West Nile virus (WNV) NS2B-NS3 protease and viral propagation by palmatine, a chemical compound from Coptis chinensis Franch. It was demonstrated that palmatine could inhibit WNV NS2B-NS3 protease activity in an uncompetitive manner, with a 50% inhibitory concentration (IC(50)) of 96 microM. Palmatine suppressed WNV without detectable cytotoxicity (a 50% effective concentration [EC(50)] of 3.6 microM and a 50% cytotoxicity concentration [CC(50)] of 1,031 microM). Furthermore, palmatine could also suppress dengue virus and yellow fever virus in a dose-dependent manner. This compound could potentially be developed for the treatment of flavivirus infections.
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