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Updated: Jun 12, 2026

Murine Model of CD40-activation of B cells
12:24

Murine Model of CD40-activation of B cells

Published on: March 5, 2010

CD40 expression in Wehi-164 cell line.

Mohammad Hossein Karimi1, Padideh Ebadi, Ali Akbar Pourfathollah

  • 1Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Karimimh@sums.ac.ir.

Cytotechnology
|May 25, 2010
PubMed
Summary
This summary is machine-generated.

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The Wehi-164 cell line shows high CD40 mRNA and cytoplasmic protein expression but lacks surface expression. This suggests the cells down-regulate surface CD40 to evade the immune system.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cancer Research

Background:

  • CD40-CD154 interaction is crucial for immune regulation and anti-tumor responses.
  • Understanding CD40 expression is vital for developing effective immunotherapies.

Purpose of the Study:

  • To investigate the expression levels of CD40 in the Wehi-164 cell line.
  • To determine if CD40 is expressed on the cell surface, in the cytoplasm, or at the mRNA level.

Main Methods:

  • Flow cytometry was used to assess cell surface CD40 expression.
  • Intracellular staining assay evaluated cytoplasmic CD40 protein levels.
  • Real-time PCR identified CD40 mRNA expression.

Main Results:

  • High mRNA expression of CD40 was detected.

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13:27

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Published on: October 16, 2009

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  • Significant cytoplasmic protein expression of CD40 was observed.
  • No detectable CD40 expression was found on the cell surface.

    • Conclusions:

    • The Wehi-164 cell line exhibits high intracellular CD40 expression.
    • The absence of surface CD40 suggests a mechanism for immune evasion.
    • Further research into this phenomenon could inform cancer immunotherapy strategies.